Spectrometry & Spectroscopy
Fluorescence Spectrophotometer - Agilent Technologies Cary Eclipse
The Cary Eclipse Spectrophotometer uses a Xenon flash lamp to measure the emission of light from samples in four modes. With superior sensitivity, high signal-to-noise ratio and fast kinetics, it captures a data point every 12.5 ms and scans at 24,000 nm/min without peak shifts.
Features
- Fluorescence, phosphorescence, chemiluminescence and bioluminescence modes
- Light source: Xenon flash lamp
- Lamp pulse width at half peak height ~2 µs, peak power equivalent to 75 kW
- Limit of detection:
- 1.0 picomolar fluorescein (< 500 µL sample volume) using a standard 10 mm cuvette
- 10 picomolar fluorescein (< 40 µL sample volume) using a 40 µL micro-cuvette
- Separate monochromators for excitation and emission
- Wavelength range: 200 – 900 nm for both excitation and emission monochromators
- Wavelength accuracy: ±0.5 nm at 541.2 nm and ±1.0 nm from 200 – 900 nm
- Wavelength reproducibility: ±0.2 nm
- Spectral band pass - Excitation: 1.5, 2.5, 5, 10, 20 nm; Emission: 1.5, 2.5, 5, 10, 20 nm
- High sensitivity: red-sensitive photomultiplier tube detectors extend to 900 nm
- Fast kinetics: scan speed of 24,000 nm/min without peak shifts and up to 80 data points per second in steady-state fluorescence mode
- Data point capture capability:
- One phosphorescence data point every 1 µs up to 10 s
- One fluorescence data point every 12.5 ms up to 999 s
- One bio/chemiluminescence data point every 40 µs up to 10 s
- Build-in excitation and emission filters to exclude secondary light
- Room light immunity for most fluorescent samples
- Cary Eclipse Control Software
Location
Materials Characterization Laboratory
Furnas Hall room #109/110
University at Buffalo North Campus
Buffalo, NY 14260
Fees
Category B Rates
- Internal Academic: $10 per hour
- External Academic: $18 per hour
- Industry: $40 per hour
Fluorescence Spectrophotometer - Agilent Technologies Cary Eclipse
For general inquiries, contact:
Donald J. Goralski
Director, Shared Instrumentation Laboratories
(716) 645-5151
For technical inquiries, contact:
Zongmin (Shirley) Bei, PhD
Senior Research Support Specialist
Shared Instrumentation Laboratories (716) 645-5165